Fig. 2: The effect of ChaC1 overexpression on AUR-induced cell death requires its gamma-glutamylcyclotransferase activity. | Cell Death Discovery

Fig. 2: The effect of ChaC1 overexpression on AUR-induced cell death requires its gamma-glutamylcyclotransferase activity.

From: ChaC1-based drug screenings identify a synergistic lethal effect of auranofin and proteasome inhibitors in hepatocellular carcinoma cells

Fig. 2

A, B Huh7 (A) and Hep3B (B) cells were infected with 1×108 pfu/mL Ad-NC, Ad-ChaC1 or Ad-ChaC1-Mut for 24 h, and then treated with 0.3 µM AUR for 12 h. The expression levels of ChaC1 were determined by western blotting. C, D Intracellular glutathione levels were measured by Tietze’s recycling assay in Huh7 (C) and Hep3B (D) cells. E, F Cell death was measured by propidium iodide staining (1 µg/mL) followed by flow cytometric analysis in Huh7 (E) and Hep3B (F) cells.

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