Fig. 5: Transcriptomic analyses from iPSC-DSN treated with cytotoxic drug and SP600125.
From: c-Jun inhibition mitigates chemotherapy-induced neurotoxicity in iPSC-derived sensory neurons
A Spearman correlation bar chart of JUN gene expression with the expression of selected genes (criteria, see below) in PTX/VCR/BTZ/CDDP- and vehicle-treated iPSC-DSN. mRNA expression levels of selected genes relative to vehicle from iPSC-DSN treated with B 100 nM PTX for 48 h, C 50 nM VCR for 16 h, D 10 nM BTZ for 16 h, or E 10 µM CDDP for 16 h, when SP600125 is present or absent (n = 3). Genes were sorted and selected based on overall Spearman correlation coefficient with reference to JUN, |R|, above 0.65, and based on whether they fulfilled the criterion of being differentially expressed with approximate absolute log2 fold change greater than 0.5 (RLD[drug] – RLD [mean vehicle] >0.5) in at least 3 out of 4 neurotoxic drugs. In A, 31 samples were included (drug and vehicle treated cells). In B–E, triplicates per condition were factored into the statistical analysis using the Mann–Whitney U test. Error bar represents the standard deviation of the mean.
