Fig. 3: Downregulation of K-Ras mediates sulindac sulfide (SS)-induced inhibition of NIH/3T3 cell transformation. | Cell Death Discovery

Fig. 3: Downregulation of K-Ras mediates sulindac sulfide (SS)-induced inhibition of NIH/3T3 cell transformation.

From: Sulindac sulfide suppresses oncogenic transformation through let-7b-mediated repression of K-Ras signaling

Fig. 3

A qRT-PCR analysis of H-Ras and K-Ras mRNA levels in transformed cells treated with 50 µM SS or vehicle control. B Immunoblot showing H-Ras and K-Ras protein levels in SS-treated and control cells. C Time-course immunoblot analysis of K-Ras expression at various stages during MCA/TPA-induced transformation. D Immunoblot detection of K-Ras expression in CRISPR/Cas9-edited K-Ras knockdown and wild-type NIH/3T3 cells. E Representative images of foci formation in wild-type and K-Ras knockdown cells under different treatment conditions (n = 5). F Quantification of foci per dish across groups. Significance levels were annotated as follows: P < 0.05 (*), P < 0.01 (**), P < 0.001 (***). Error bars represent mean ± s.d.

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