Fig. 2: GSDMD knockout attenuates pulmonary macrophage mobilization and infiltration during BPD development.
From: GSDMD deficiency attenuates BPD by suppressing macrophage pyroptosis and promoting M2 polarization
A Granulocyte counts in pups from the indicated treatment groups were quantified. B Macrophage numbers were determined in the same samples. C Lymphocyte levels were measured for comparison. Data are presented as mean ± SD of multiple independent experiments; ****P < 0.0001 (one-way ANOVA). D Representative F4/80 immunostaining identifying macrophages in lung sections from pups in the indicated groups. For each sample, 3–4 random fields were captured at 20× magnification; scale bar = 50 µm. The percentage of F4/80⁺ macrophages was quantified (n = 5 per group). Columns show mean ± SD of multiple independent experiments; ****p < 0.0001 (one-way ANOVA). E Immunofluorescence co-localization images of GSDMD (red) and DAPI (blue, nuclear stain) in lung tissue. Images were acquired at 20× magnification; scale bar = 50 µm. F Concentrations of chemokines CXCL2 and CCL3 in lung homogenates from mice in the indicated groups (n = 5 per group). Data are expressed as mean ± SD of multiple independent experiments; ****P < 0.0001 (one-way ANOVA).
