Fig. 5: Effects of SIRT1 inhibitor and SIRT6 inhibitor on aortic calcification in CKD mice.
From: Niacin inhibits vascular calcification via modulating of SIRT1/SIRT6 signaling pathway
Adenine diet-induced CKD mice were treated with niacin (600 mg/kg) together with EX527 (5 mg/kg/d, i.p.) or OSS_128167 (10 mg/kg/d, i.p.) for 9 weeks (n = 8 for each group). A Scheme of the construction of the CKD-associated vascular calcification model and EX527 or OSS_128167 supplement. B Western blots for the expression of SIRT1 and SIRT6 of aortic arteries. C Representative images of aortic calcification (red arrows) are shown after examining by micro-CT. D Mineral deposition was detected by alizarin red staining of whole mount of aorta. Representative images showing aortic arteries staining with Alizarin red. E and F Representative images of von Kossa and Alizarin red staining of aortic sections. Scale bar = 50 μm. G Representative western blot analysis and quantification of Runx2 and OPN protein levels in arteries from different groups. Data are shown as mean ± SEM. * P < 0.05, ** P < 0.01. One-way ANOVA, Tukey’s HSD post hoc test.
