Fig. 5: O-GlcNAcylation of Raptor modulates mTORC1-mediated glycolytic enhancement.
From: LBX2 promotes colorectal cancer progression via the glycosylation and lactylation positive feedback
A Western blot analysis of p-Akt/mTOR pathway activation following LBX2 knockdown and/or UDP-GlcNAc disodium supplementation (250 μM for 24 h) in SW480 cells; B Western blot analysis of SW480 cells with LBX2 knockdown and/or UDP-GlcNAc disodium supplementation (250 μM for 24 h), transfected with HA-Raptor plasmid, and subjected to immunoprecipitation using anti-HA antibody; C Conservation of residues 681–721 in Raptor. Pink: T700; D Structure of human Raptor-Rag (PDB: 6U62). Residue T700 is in a flexible region (693–776 aa) of Raptor; E Western blot analysis of SW480 cells with LBX2 knockdown, transfected with wild-type or T700D mutant HA-Raptor plasmid, and subjected to immunoprecipitation using anti-HA antibody; F The relative phosphorylation level of S6K in SW480 cells with LBX2 knockdown, transfected with wild-type or T700D mutant HA-Raptor plasmid, was calculated by quantifying the ratio of p-S6K to total S6K band intensity; G, H Western blot analysis of SW480 cells with LBX2 knockdown, transfected with HA-Raptor plasmid, and subjected to immunoprecipitation using anti-HA antibody; I ELISA analysis of intracellular 2-DG levels following LBX2 knockdown in SW480 cells; J Measurement of extracellular acidification rate (ECAR) in SW480 cells with LBX2 knockdown; K ELISA analysis of intracellular lactate levels following LBX2 knockdown in SW480 cells. *P < 0.05; **P < 0.01; ****P < 0.0001.
