Fig. 2: Hypoxia-induced autophagy contributed to DDP resistance in NSCLC cells. | Cell Death Discovery

Fig. 2: Hypoxia-induced autophagy contributed to DDP resistance in NSCLC cells.

From: Hypoxia-triggered autophagy modulates cisplatin resistance in non-small cell lung Cancer via EIF2AK3-dependent PI3K/AKT signaling and mTOR-independent mechanisms

Fig. 2

A549 and 95D cells were treated with 3-MA (10 mM) and then exposed to hypoxia for 24 h. A Representative immunofluorescence images of LC3 in A549 and 95D cells. B Protein expression of the LC3-II/I ratio and corresponding quantitative analyses in A549 and 95D cells. C Cell viability measured by CCK-8 assay in A549 and 95D cells treated with different concentrations of DDP under normoxia, hypoxia, or hypoxia + 3-MA for 24 h. D Apoptosis assessed by flow cytometry with Annexin V-FITC/PI staining in A549 and 95D cells treated with DDP (2 µg/ml) under normoxia, hypoxia, or hypoxia + 3-MA for 24 h. Data are presented as mean ± SD from three independent experiments. **p < 0.01, ***p < 0.001 versus normoxia; ##p < 0.01, ###p < 0.001 versus hypoxia.

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