Fig. 6: PFKFB3 protects cartilage from post-traumatic osteoarthritis in vivo.
From: The glycolytic enzyme PFKFB3 alleviates DNA damage and chondrocyte senescence in osteoarthritis
a Safranin O-Fast Green (SO&FG) staining, immunofluorescence (IF) staining of PFKFB3, p16INK4a, and p21, immunohistochemical (IHC) staining of COL2A1, MMP13, p-ATM, and p-ATR in the cartilage of mice subjected to 8 weeks post-DMM surgery and administered with AAV-PFKFB3 via intra-articular injection. b Osteoarthritis Research Society International (OARSI) scores and quantification of TUNEL, PFKFB3, COL2A1, p-ATM, and p-ATR signals in the cartilage of mice subjected to DMM surgery and injected with AAV-PFKFB3. Data presented as means ± s.e.m., n = 6, one-way ANOVA with Tukey’s multiple comparisons. c SO&FG staining, IF staining of p16INK4a, p21, and PFKFB3, and IHC staining of COL2A1 in the cartilage of mice administered with AAV-shPFKFB3 via intra-articular injection. d OARSI scores and quantification of p16INK4a, p21, PFKFB3, and COL2A1 signals in the cartilage of mice injected with AAV-shPFKFB3. Data presented as means ± s.e.m., n = 6, unpaired Student’s t-test. *P < 0.05, **P < 0.01. ns not significant.
