Fig. 2: PREP knockout prevents bone loss induced by ovariectomy. | Cell Death Discovery

Fig. 2: PREP knockout prevents bone loss induced by ovariectomy.

From: Silencing of prolyl endopeptidase protects against bone loss and enhances regeneration via bone anabolic and anti-catabolic effects

Fig. 2

A Three-dimensional reconstruction of the distal femur by micro-CT. B Silver-stained images of the distal femur in sham-operated, wild-type and RREP knockout osteoporotic model mice. C, D Comparison of BMD, Tb.Sp, Tb.N, BV/TV, and Tb.Th between sham-operated, wild-type and RREP knockout osteoporotic model mice. E, F Reconstruction images of cortical bone and comparison of cortical bone thickness between sham-operated, wild-type and RREP knockout osteoporotic model mice. G, H HE and Safranin O/Fast Green staining of the femur in sham-operated, wild-type and RREP knockout osteoporotic model mice. Scale bars = 100 μm. I Double-labeling with calcein and alizarin red in sham-operated, wild-type and RREP knockout osteoporotic model mice. Scale bars = 10 μm. J Immunohistochemical staining of Runx2 and quantification of osteoblast numbers in the distal femur of sham-operated, wild-type, and RREP knockout osteoporotic model mice. Scale bars = 100 μm. K Comparison of MAR between sham-operated, wild-type, and RREP knockout osteoporotic model mice. L TRAP staining and quantification of osteoclast numbers in the distal femur of sham-operated, wild-type, and RREP knockout osteoporotic model mice. Scale bars = 100 μm.

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