Fig. 4: Osteoblastogenesis of BMSCs is inhibited in vitro by PREP. | Cell Death Discovery

Fig. 4: Osteoblastogenesis of BMSCs is inhibited in vitro by PREP.

From: Silencing of prolyl endopeptidase protects against bone loss and enhances regeneration via bone anabolic and anti-catabolic effects

Fig. 4

A Alizarin Red and ALP staining of BMSCs from wild-type and PREP knockout mice after osteogenic differentiation. B PCR analysis of the expression of osteogenic differentiation marker genes RUNX2, OSTERIX, OCN, and ALP in BMSCs from wild-type and PREP knockout mice. C Measurement of ALP activity in BMSCs from wild-type and PREP knockout mice. D Western blot analysis of the protein expression changes in osteogenic differentiation-related genes RUNX2, OSTERIX, OCN, and ALP in BMSCs from wild-type and PREP knockout mice. E Flow cytometry shows that PREP knockout does not affect apoptosis in BMSCs. F Alizarin Red and ALP staining of BMSCs with normal and overexpressed PREP after osteogenic differentiation. G PCR analysis of the expression of osteogenic differentiation marker genes RUNX2, OSTERIX, OCN, and ALP in BMSCs with normal and overexpressed PREP. H Measurement of ALP activity in BMSCs with normal and overexpressed PREP. I Western blot analysis of the protein expression changes in osteogenic differentiation-related genes RUNX2, OSTERIX, OCN, and ALP in BMSCs with normal and overexpressed PREP. J Flow cytometry shows that overexpression of PREP does not affect apoptosis in BMSCs. n = 5, *P < 0.05, **P < 0.01 and ***P < 0.001 by t test.

Back to article page