Fig. 6: Osteoclastogenesis process is affected by PREP. | Cell Death Discovery

Fig. 6: Osteoclastogenesis process is affected by PREP.

From: Silencing of prolyl endopeptidase protects against bone loss and enhances regeneration via bone anabolic and anti-catabolic effects

Fig. 6

A TRAP staining to assess the impact of PREP knockout or knockdown on osteoclast differentiation in mouse bone marrow mononuclear cells and raw264.7 cell line. Scale bars= 50 μm. B Quantification of the number of osteoclasts. C PCR analysis of the mRNA expression of osteoclast differentiation-related genes in mouse bone marrow mononuclear cells and raw264.7 cell line after PREP knockout or knockdown. D Fluorescent staining with phalloidin to show that PREP knockdown inhibits cell fusion in raw264.7 cells. Scale bars = 20 μm. E Western blotting to investigate osteoclast differentiation-related proteins in mouse bone marrow mononuclear cells and raw264.7 cell line after PREP knockout or knockdown. F TRAP staining to assess the impact of PREP overexpression on osteoclast differentiation in mouse bone marrow mononuclear cells and raw264.7 cell line. Scale bars = 50 μm. G Quantification of the number of osteoclasts formed with PREP overexpression. H PCR analysis of the mRNA expression of osteoclast differentiation-related genes in mouse bone marrow mononuclear cells and raw264.7 cell line after PREP overexpression. I Fluorescent staining with phalloidin to show that PREP overexpression promotes cell fusion in raw264.7 cells. Scale bars = 20 μm. J Western blotting to investigate osteoclast differentiation-related proteins in mouse bone marrow mononuclear cells and raw264.7 cell line after PREP overexpression. K, L Bone resorption assays indicating that PREP enhances osteoclasts’ bone resorptive capability. Scale bars = 20 μm. n = 5, *P < 0.05, **P < 0.01 and ***P < 0.001 by t test.

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