Fig. 6: MT promoted the DAPCs differentiation potential of hiPSCs by regulating MT receptor MT1/2. | Cell Death Discovery

Fig. 6: MT promoted the DAPCs differentiation potential of hiPSCs by regulating MT receptor MT1/2.

From: Melatonin orchestrates mitochondrial fusion dynamics-mediated WNT/β-catenin signaling to promote dopaminergic neuronal differentiation of human iPS and nerve regeneration in a MPTP-induced mouse model of Parkinson’s disease

Fig. 6

A, B Immunofluorescence staining of NSC markers (Nestin, Pax6, SOX1) in NSCs and differentiated DAPCs. Scale bars, 50 μm. C Immunofluorescence staining of the midbrain neural marker Nurr1 in NSCs and differentiated DAPCs. Scale bars, 50 μm. D Flow cytometry analysis of NSC markers (Nestin, Pax6, SOX1) and the midbrain neural marker Nurr1. E Immunofluorescence staining of DA neuron markers (TH, EN1) in NSCs and differentiated DAPCs. Scale bars, 50 μm. F PCA analysis from RNA-Seq among NSCs and DAPCs before and after 10 μM MT treatment. G, H Gene ontology (GO) and Gene Set Enrichment Analysis (GSEA) from RNA-seq between DAPCs MT 0 μM and DAPCs MT 10 μM. I Heat map view of selected gene sets associated with neurons and synapses from RNA Seq of DAPCs MT 0 μM and DAPCs MT 10 μM. (J) Immunofluorescence staining of MFN2 in the differentiated DAPCs. Scale bars, 50 μm. K Immunofluorescence staining of MFN2 in the differentiated DA neurons. Scale bars, 50 μm.

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