Fig. 2: High UCHL1 expression is positively correlated with EMT and migration in NSCLC cells. | Cell Death Discovery

Fig. 2: High UCHL1 expression is positively correlated with EMT and migration in NSCLC cells.

From: UCHL1 stabilizes Twist1 via K11/K63-linked deubiquitination to drive tumor metastasis in non-small cell lung cancer

Fig. 2

A Western blot analysis was performed to assess UCHL1 and EMT marker expression in NSCLC cell lines. B The migration of NSCLC cells was evaluated using a wound-healing assay. Red bar, 200 μm. C Cell invasion was measured in a transwell invasion assay. Red bar, 100 μm. D EMT marker protein levels were analyzed in H1299 cells treated with shUCHL1 or shNC lentivirus by western blotting. E EMT marker protein levels were assessed in A549 cells transfected with Vector or Flag-UCHL1 plasmids by western blotting. F Cell migration was determined using wound-healing assays. Red bar, 200 μm. G Cell invasion was evaluated in a transwell invasion assay. Red bar, 100 μm. In (B, C, F, and G), Mann–Whitney test (n = 3, independent experiments), *p < 0.05, **p < 0.01.

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