Fig. 3: Characterization of human lung organoids (hLORGs) derived by hiPSCs TLR3W769*.
From: Modelling severe COVID-19 in TLR3-mutated hiPSCs-derived lung organoids
A Phase–contrast microscopy of alveolospheres embedded in 3D Matrigel at day 51 of culture. Scale bar 200 µm. B Haematoxylin and eosin-stained hLORG cross-section showing the typical epithelial morphology. Scale bar 50 µm. C–E ACE2, SFTPC protein and gene expression in hLORGs at day 51 of differentiation by immunohistochemistry and qPCR analysis. F FOXA2 protein expression in hLORGs at day 51 of differentiation by immunohistochemistry. Scale bar 50 µm. G DPP4 protein expression in hLORGs at day 51 of differentiation by immunofluorescence. Scale bar 50 µm. H, I Immunofluorescence images show the overall actin (red) and α-tubulin distribution (green) of an hLORG displaying a prominent epithelial structure (nuclei, blue). Scale bar 50 µm and 25 µm, respectively.
