Fig. 1: WWP1 GOF induced neurodevelopmental defects.
From: WWP1 gain-of-function drives developmental anoikis through TGFβ pathway during neurodevelopment
A Representative images of E18.5 brain sections electroporated with control, WWP1OE, WWP1E798V, WWP1E798V C890A constructs. Green, GFP; red, CTIP2; blue, DAPI. Dashed lines separated cortex zones into upper cortical plate (uCP), middle cortical plate (mCP), and lower cortical plate/subventricular zone/ventricular zone (loCP/SVZ/VZ). Scale bar, 50 μm. B Quantification of GFP+ cell distribution across cortical layers. n = 5 per group. Two-way ANOVA with Tukey’s post hoc test. *p < 0.05; **p < 0.01; ****p < 0.0001. C Representative images of E15.5 brain sections electroporated with control or WWP1 variants. Green, GFP; red, CC3; blue, DAPI. Scale bar, 50 μm. D Quantification of CC3+ cells in the electroporated region. Control, n = 10; WWP1OE, n = 7; WWP1E798V, n = 9; WWP1C890A, n = 6; WWP1E798V C890A, n = 7. Kruskal-Wallis test with Dunn’s post hoc test. **p < 0.01; ***p < 0.001. E Representative images of E15.5 brain sections electroporated with control or WWP1E798V constructs administered with vehicle or Z-VAD-FMK. Green, GFP; red, CC3; blue, DAPI. Scale bar, 50 μm. F, G. Quantification of total CC3+ (F) and CC3+ among GFP+ cells (G) in the electroporated region. Control with vehicle, n = 6; control with Z-VAD-FMK, n = 5; WWP1E798V with vehicle, n = 7; WWP1E798V with Z-VAD-FMK, n = 7. Kruskal-Wallis test with Dunn’s post hoc test. *p < 0.05; **p < 0.01. Bar graphs indicate mean ± SEM.
