Fig. 2: BACH2 binds to conserved regions of the MIAT promoter. | Cell Death Discovery

Fig. 2: BACH2 binds to conserved regions of the MIAT promoter.

From: BACH2 links β1-adrenergic receptor/β-arrestin1 signaling to MIAT to inhibit cardiac fibroblast activation and cardiomyocyte apoptosis

Fig. 2: BACH2 binds to conserved regions of the MIAT promoter.

A Sequence homology and conserved BACH2 binding regions (E1 and E2) within human and mouse MIAT promoters. Potential BACH2 binding sites are shown as red fonts. B Potential BACH2-binding sites conserved between human and mouse MIAT promoters. The binding site is shown only when the recognition sequence is found in both human and mouse MIAT promoters. C, D Electrophoretic mobility shift assays (EMSAs) were performed with oligonucleotides containing two conserved BACH2-binding regions (E1 and E2) in the human promoter. DNA-BACH2 protein complexes are markedly decreased by the addition of BACH2 antibodies, whereas the formation of specific BACH2-DNA complexes (shown with top blue arrow in C) is not inhibited by IgG antibodies. One-way ANOVA with Tukey’s multiple comparison test. *P < 0.05, **P < 0.01, or ***P < 0.001 vs. control: DC or PC. #P < 0.05 or ##P < 0.01 vs. - or IgG.

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