Fig. 5: GQ20-PTO inhibits virus replication in ALI cultures of primary human bronchial epithelial (HBE) cells. | Cell Death Discovery

Fig. 5: GQ20-PTO inhibits virus replication in ALI cultures of primary human bronchial epithelial (HBE) cells.

From: Discovery of synthetic G-quadruplex DNA as SARS-CoV-2 helicase inhibitor with antiviral, anti-inflammatory and antioxidative properties

Fig. 5: GQ20-PTO inhibits virus replication in ALI cultures of primary human bronchial epithelial (HBE) cells.

A Schematic display of the experimental set-up. B Immunoblot analysis of viral nucleocapsid protein (NP) in the presence of GQ20-PTO. C Densitometric analysis of NP. Signals were normalized to GAPDH and related to the virus control (ctrl). Statistical analysis was performed in relation to virus control using one-way ANOVA with P values indicated in the graph. D Quantitative levels of virus-induced caspase 3/7 activation in the presence of GQ20-PTO. P values of one-way ANOVA are indicated. E Quantitative measurement of the transepithelial electrical resistance (TEER) after virus infection in the presence of GQ20-PTO. P values of one-way ANOVA are indicated in the graph. F Immunoblot analysis of interferon (IFN) signaling of infected and treated ALI cultures. G Densitometric analysis of pSTAT, Mx1 and ISG15 shown in (F). P values of one-way ANOVA are indicated in the graph. H Representative images of HBE ALI cultures treated with (1 µM) Cy3-labeled GQ20-PTO (applied apically and to the basal medium) for 24 h; Cy3 in orange, Hoechst 33324 in blue. Scale bar: 50 µm.

Back to article page