Fig. 5: FUT8 regulates pan-lysine lactylation and promotes PKM2 lactylation in ccRCC. | Cell Death Discovery

Fig. 5: FUT8 regulates pan-lysine lactylation and promotes PKM2 lactylation in ccRCC.

From: FUT8 reprograms glycolytic metabolism to promote PKM2 lactylation and drive clear cell renal cell carcinoma progression

Fig. 5: FUT8 regulates pan-lysine lactylation and promotes PKM2 lactylation in ccRCC.

A, B pan-lysine lactylation (Pan-Kla) levels in 786-O and Caki-1 cells after 24 h exposure to graded concentrations of NaLac or NaOxa. C Western blot analysis of pan-Kla in FUT8-knockdown versus control 786-O and Caki-1 cells. D, E Analysis of pan-Kla levels and FUT8 expression in human ccRCC tumor tissues. Representative Western blots of pan-Kla and FUT8 in tumor samples (n = 13) (D), and correlation analysis between relative FUT8 and pan-Kla protein levels (E). F–H Lactyl-proteomic profiling of FUT8-regulated lactylation. Bar charts show the numbers of upregulated and downregulated lactylated proteins and modification sites (F). Gene Ontology (GO) enrichment analysis of FUT8-regulated lactylated proteins (G). Subcellular localization distribution of FUT8-regulated lactylated proteins (H). I Mass-spectrometric identification of PKM2 lysine 115 (K115) lactylation. J Immunoprecipitation of PKM2 followed by immunoblotting with anti-pan-Kla antibody in control and FUT8-knockdown 786-O cells, confirming reduced PKM2 lactylation upon FUT8 depletion.

Back to article page