Fig. 2: Sublethal DNA damage suppresses IL-10 and IFN-γ secretion more strongly than it reduces cell survival.
From: Sublethal DNA damage switches off B cell effector programs in an RA-FLS-PBMC co-culture
Day 8 (7 days post-treatment) supernatant dose/concentration responses for IL-10 (A–C) and IFN-γ (D–F) in PBMC-RA-FLS co-cultures (red), plotted against the matched day 8 viability (black) of the PBMC-RA-FLS co-cultures from Fig. 1 for γ-IR (A, D), 4-OOH IFA (B, E), and H₂O₂ (C, F). Values are expressed as % of CpG-stimulated untreated control. The viability curve (black line, secondary y-axis) is identical across all panels and represents day 8 viability from the same wells used for the corresponding supernatant measurements. Spearman correlations with viability are summarized in Supplementary Table S4. Statistics: repeated-measures one-way ANOVA with Dunnett’s post hoc vs untreated control. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001; ns not significant. PBMC-RA-FLS: N = 3, n = 3.
