Fig. 4: γ-H2AX kinetics reveal lineage-specific DNA damage burdens at IC₂₀ versus IC₅₀.
From: Sublethal DNA damage switches off B cell effector programs in an RA-FLS-PBMC co-culture
IC₂₀ (A–C) and IC₅₀ (D–F) time courses (2, 8, 16, 24 h) of γ-H2AX median fluorescence intensity (MFI) in gated CD4⁺ T, CD8⁺ T, memory B (CD19⁺CD27⁺), and naïve B (CD19⁺CD27−) cells within PBMC-RA-FLS co-cultures after γ-IR (A, D), 4-OOH IFA (B, E), or H₂O₂ (C, F). MFI is normalized to the time-matched untreated control. IC₂₀/IC₅₀ doses/concentrations correspond to those derived in Fig. 1 (Supplementary Table S2). Gating strategy: Supplementary Fig. S3; Statistics: repeated-measures one-way ANOVA with Dunnett’s post hoc vs time-matched control at each timepoint. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001; ns not significant. Adjusted p-values for comparisons to the 0-h baseline within each lineage are reported in Supplementary Table S5. PBMC-RA-FLS: N = 4, n = 1.
