Fig. 5: Sublethal genotoxic stress imposes distinct cell cycle checkpoints in memory versus naïve B cells.
From: Sublethal DNA damage switches off B cell effector programs in an RA-FLS-PBMC co-culture
Ki-67-FITC/Propidium iodide (PI) profiles at 24 h after the single IC₂₀ or IC₅₀ pulse (A–C) and at 48 h (i.e., 24 h after CpG stimulation; D-F). For each time point, the three stressors are shown side by side: γ-IR (A, D), 4-OOH IFA (B, E), and H₂O₂ (C, F). Within each stressor, the left-hand bars represent memory B cells, and the right-hand bars represent naïve B cells gated within PBMC-RA-FLS co-cultures. Stacked columns display mean ± SEM percentages of cells in G₀ (blue), G₁ (green), and S-G₂-M (red) phases, illustrating checkpoint-mediated accumulation in G₁ with a smaller fraction of cells arrested in S-G₂-M after sublethal genotoxic stress. IC₂₀/IC₅₀ doses/concentrations are as defined in Fig. 1 (Supplementary Table S2). Statistics: repeated-measures one-way ANOVA with Dunnett’s post hoc vs untreated control. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001; ns not significant. PBMC-RA-FLS: N = 4, n = 1.
