Fig. 6: Early transcriptional wiring of the damage response at 24 h.
From: Sublethal DNA damage switches off B cell effector programs in an RA-FLS-PBMC co-culture
Bar charts show mRNA fold change (ΔΔCt) relative to time-matched untreated control of the whole PBMC-RA-FLS co-culture, measured 24 h after a single IC₂₀ or IC₅₀ dose/concentration of γ-IR (A, D), 4-OOH IFA (B, E), or H₂O₂ (C, F) (mean ± SEM). Upper row (A–C): DNA damage sensors and repair genes (ATM, APEX1, XRCC6, XRCC5). Lower row (D–F): apoptosis regulators (FAS/FASLG, BAX, BCL2). IC₂₀/IC₅₀ doses/concentrations follow the viability curves in Fig. 1 (Supplementary Table S2). Statistics: repeated-measures one-way ANOVA with Dunnett’s post hoc vs control. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001; ns not significant. PBMC-RA-FLS: N = 3, n = 2.
