Fig. 4: Analysis of cell death in DADA2 after inhibition of regulated cell death pathways.
From: ADA2-deficient cells exhibit increased levels of cell death and metabolic disturbances
Cell death was measured by flow cytometric measurement of staining with Annexin V and Zombie® viability dye. Apoptosis, necroptosis and pyroptosis were inhibited by incubation with 20 µM Z-VAD-FMK (Z-VAD), 10 µM necrostatin-1s (Nec-1s) and 20 µM Z-YVAD-FMK (Z-YVAD), respectively, for the indicated periods of time. A Data from n = 12 | 12 | 8 (HC) and n = 8 | 8 | 5 (DADA2) independent experiments for Z-VAD|Nec-1s|Z-YVAD, respectively, are shown. B The experiment was performed for n = 2 distinct ADA2-/- U-937 cell clones. C Data from n = 5 (HC) and n = 4 (DADA2) independent experiments are shown. D Ferroptosis was inhibited by 48-hour incubation with 50 µM deferoxamine (DFO) or 2 µM ferrostatin-1 (Fer-1) in n = 2 HC and DADA2 patients. The median is indicated in all plots. Mann-Whitney-U test, *p < 0.05, **p < 0.01. The patients’ phenotype is indicated by colour as displayed in Fig. 1B.
