Fig. 8: Pentose phosphate pathway and oxidative stress in DADA2.
From: ADA2-deficient cells exhibit increased levels of cell death and metabolic disturbances
A Analysis of gene expression by single-cell RNA sequencing in HC and DADA2 monocytes based on the data published by Watanabe et al. (GSE142444) [11]. Heatmap showing the normalised log reads (z-scores) of the genes associated with the pentose phosphate pathway (reactome id R-HSA-71336). Of these, PGD and RBKS show a significant difference at logFC = −0.37, FDR = 0.03 and logFC = −2.35, FDR = 0.02 respectively. B Schematic overview of the reactions of the pentose phosphate pathway. C Total content of reactive oxygen species was measured by flow cytometry in ADA2WT/WT and ADA2-/- Jurkat cells at baseline and after incubation with H2O2. D Total content of reactive oxygen species was measured by flow cytometry in PBMCs from healthy controls (HC) and DADA2 patients P5 and P6 at baseline and after incubation with H2O2. P6 had undergone successful HSCT at the time of sampling. Legend: 6PGD 6-phosphogluconate dehydrogenase, 6PGL 6-phosphogluconolactonase, G6PD glucose-6-phosphate dehydrogenase, gMFI geometric mean fluorescence intensity, ROS reactive oxygen species.
