Fig. 6: ATP6V1B2 promotes autophagic degradation of FASN protein.
From: ATP6V1B2 alleviates hepatic steatosis by promoting lysosomal acidification in hepatocytes
A Western blot detection of P62, LC3B expression, and quantification in OPA-treated HepG2 transfected with pCtr or pATP6V1B2. B Western blot detection of P62, LC3B protein expression, and quantification in HepG2-shCtr and HepG2-shATP6V1B2 cells. C Western blot to detect the expression of FASN, P62, and LC3B proteins in MG132 (20 µM)-treated HepG2-shCtr and HepG2-shATP6V1B2 cells. D Western blot detection of FASN protein expression in CQ (20 µM)-treated HepG2-shCtr and HepG2-shATP6V1B2 cells. E Immunofluorescence detection of FASN and LC3B expression in MG132 (20 µM)-treated HepG2 cells transfected with siCtr and siATP6V1B2. (Scale bar = 20 µm). F Co-localization of FASN and LAMP1 in HepG2 cells transfected with siCtr and siATP6V1B2 detected by LysoTracker Red and immunofluorescence. (Scale bar = 20 µm). All data were expressed as the means ± SD of at least 3 independent experiments, *P < 0.05; **P < 0.01; ***P < 0.001.
