Fig. 2: CB2R knockout exacerbates macrophage pyroptosis after skeletal muscle injury. | Cell Death Discovery

Fig. 2: CB2R knockout exacerbates macrophage pyroptosis after skeletal muscle injury.

From: Cannabinoid type 2 receptor regulates skeletal muscle regeneration by NLRP3-GSDMD mediated macrophage pyroptosis after injury

Fig. 2: CB2R knockout exacerbates macrophage pyroptosis after skeletal muscle injury.The alternative text for this image may have been generated using AI.

a The average fluorescence intensity of F4/80 and GSDMD, scale bar = 20μm, n = 3, *p < 0.05 vs. WT group. b Western blot and quantitative analysis of NLRP3, GSDMD, GSDMD-NT, caspase-1 and IL-18 in each group 3 days after skeletal muscle injury, n = 3, *p < 0.05 vs. WT group, #p < 0.05 vs. Vehicle group. c PI pictures of peritoneal macrophages treated with LPS + ATP. d Immunofluorescence of caspase-1(red) and GSDMD (green), blue channel is DAPI stained nuclei, scale bar = 20 μm, n = 5, *p < 0.05 vs. WT-3d group, #p < 0.05 vs. Vehicle group. e Western blot and quantitative analysis of NLRP3, GSDMD, GSDMD-NT and caspase-1, n = 3, *p < 0.05 vs. WT group, #p < 0.05 vs. sham group. Data are represented as mean ± SD. ns no significance.

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