Fig. 4: CDH26 variant A is involved in cytoskeletal dynamics.

a HeLa cells co-expressing Empty-GFP and actin marker LifeAct mCherry show typical actin features of fibroblastoid cells. b Cells expressing CDH26-GFP have cortical actin, a feature of epithelioid cells, showing actin reorganization. Figures are representative of data from n = 3 separate experiments and four fields per condition. c Phase image for comparison of cell shape between Empty-GFP and CDH26-GFP cells—single-cell (red) selected to show shape difference between conditions. d Morphometric measurements made on stable expression in selected HeLa cells. ***p < 0.0001 and **p = 0.0028 significance, Unpaired t-test. ^†Data from single cells measured, n = 14 fields at 60×, two separate experiments. e Super-resolution confocal microscopy images showing localization of Clover-N1 and CDH26-Clover-N1 (green) to mRuby2-LifeAct (red) and CellMask Deep Red (blue). Scale bars = 10 µm, unless otherwise noted.