Fig. 2: CARK1 interacts with RCAR3 or RCAR11 in vitro and in vivo. | Cell Discovery

Fig. 2: CARK1 interacts with RCAR3 or RCAR11 in vitro and in vivo.

From: CARK1 mediates ABA signaling by phosphorylation of ABA receptors

Fig. 2: CARK1 interacts with RCAR3 or RCAR11 in vitro and in vivo.The alternative text for this image may have been generated using AI.

a GST pull-down assay of CARK1-KD and CARK1-KDN204A interaction with RCAR3 or RCAR11. His-tagged CARK1-KD or CARK1-KDN204A (the amounts with a linear increase from 50 μg, 100 μg and 200 μg) was incubated with 100 μg of GST-tagged RCAR3 or RCAR11, GST, respectively. The samples were analyzed by SDS-PAGE (top) and immunoblot (bottom), the relative protein bands were labeled by the arrow. A quantity of 0.2 μg of input was used. b, c Co-IP experiment of HA-tagged CARK1/CARK1N204A and Flag-tagged RCAR3 (b) or Flag-tagged RCAR11 (c). Immunoprecipitates against anti-HA antibody (IP) or crude extracts (Input) were analyzed against anti-HA antibody and subjected to immunoblot analysis against anti-Flag and anti-HA antibodies. Crude extracts without plasmids transfection were used as the negative control (NC). d BiFC assay with different combinations of nYFP-CARK1/CARK1N204A and cYFP-RCAR3/RCAR11 in the leaves of N. benthamiana. Pictures were taken using a confocal laser scanning microscope (LSM 710, Carl Zeiss). Scale bar = 100 µm

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