Fig. 5: RPS3A knockdown in brown preadipocyte suppresses adipogenesis. | Cell Discovery

Fig. 5: RPS3A knockdown in brown preadipocyte suppresses adipogenesis.

From: RPS3A positively regulates the mitochondrial function of human periaortic adipose tissue and is associated with coronary artery diseases

Fig. 5: RPS3A knockdown in brown preadipocyte suppresses adipogenesis.

a Brown preadipocytes were treated with RNAi before adipogenic induction and collected for qPCR to determine relative mRNA expression levels of RPS3A (n = 3). b Oil Red O staining of adipocytes differentiated from brown preadipocytes on day 8. c qPCR to determine the decreased mRNA expression of adipocyte markers (n = 3). d and e Flow cytometric analysis of EdU+ cells in brown preadipocytes (n = 3). f Brown preadipocytes were treated with RNAi before adipogenic induction and Western blot experiment using antibodies against C/EBPβ, PPARγ, aP2, and Actin on day 4

Back to article page