Fig. 7: RPS3A-regulated PVAT plays an important role in atherosclerosis in vivo. | Cell Discovery

Fig. 7: RPS3A-regulated PVAT plays an important role in atherosclerosis in vivo.

From: RPS3A positively regulates the mitochondrial function of human periaortic adipose tissue and is associated with coronary artery diseases

Fig. 7: RPS3A-regulated PVAT plays an important role in atherosclerosis in vivo.

a Images of mouse cardiovascular following injection of adenovirus adjacent to the periaortic arch. b–e Adenovirus expressing RPS3A shRNA was injected weekly adjacent to the aortic arch for 2 weeks. LacZ shRNA was simultaneously injected as a control. b Periaortic arch adipose tissues were subjected to H&E staining and immunohistochemical staining with UCP1, RPS3A. c qPCR of mRNAs encoding brown adipocyte markers in the periaortic arch adipose tissue (n = 4). d Representative sections of aortic arch stained with H&E. e qPCR of mRNAs encoding inflammation and macrophage markers in the aorta arch (n = 4). f Adipocyte-conditioned medium was cultured with aorta from mice. g The aortic sections from control mice were incubated with CM derived from mature adipocytes, and treated with pAd-LacZ or pAd-RPS3A for 24 h; the aortic sections were harvested and qPCR of mRNAs encoding inflammation and macrophage markers were analyzed (n = 3)

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