Fig. 6: Hda1 and Esa1 regulate Eaf1 K173 acetylation via Brg1 recruitment.
From: Merge and separation of NuA4 and SWR1 complexes control cell fate plasticity in Candida albicans
a Acetylation of Eaf1 in yeast cells requires Esa1. Eaf1-Myc was introduced into wild-type or esa1 mutant cells and cultured in YPD at 25 °C for 6 h and in YPD plus 10% serum at 35 °C for 3 h. Then WCEs were collected, immunoprecipitated with anti-Myc, and probed with anti-Ac-lysine. b Deacetylation of Eaf1 in hyphal cells needs Hda1. Eaf1-Myc was introduced into wild-type, hda1, rpd3, and rpd31 mutant, respectively, and cultured in YPD at 25 °C or YPD plus 10% serum at 37 °C for Co-IP and IB. c Eaf1 is deacetylated through a Brg1-dependent mechanism. Cells of wild-type or brg1 mutant carrying Eaf1-myc were induced in YPD plus 10% serum at 37 °C and collected at time points indicated for Co-IP or IB. d Eaf1K173R bypasses the requirement of Brg1 in hyphal elongation. Yeast cells of wild-type, Eaf1K173R, Eaf1K173Q, brg1, and brg1 carrying Eaf1K173R mutant were induced at 37 °C in YPD, YPD with 10% serum, YPD with 5 nM rapamycin, YEP with 2% GlcNAc, or Lee’s with mannitol for hyphal development. The level of hyphal elongation is indicated by the number of + symbols
