Fig. 3: Cryo-EM structures of Pol III open complexes.
From: Structural visualization of RNA polymerase III transcription machineries
a Nucleic acid scaffold used. Transcription bubble position is also labeled. For the CC (closed complex) scaffold, the bubble is putative, and is labeled using a dashed line. The color scheme is the same as in Fig. 1a. b, c Cryo-EM reconstructions of the Pol III OC (b) and nOC (natural OC; c). The front view is shown, and the color scheme is the same as in Fig. 1b. d Cryo-EM reconstruction of the Pol III iOC (intermediate OC). The color scheme is the same as in Fig. 1b. The left zoom in panel shows that the density of the Brf1 ZR domain is absent in the iOC reconstruction. The iOC density is shown as transparent surface, while the Brf1 ZR domain from the OC reconstruction is shown as ribbon diagram. The right zoom in panel shows the comparison of the downstream DNA between OC and iOC. The DNA model from the OC reconstruction is shown as ribbon diagram, and the downstream DNA density in the iOC reconstruction is shown as mesh
