Fig. 5: Class-selective de-β-hydroxybutyrylation of histones H3 and H4 by SIRT3 in vitro.
From: Molecular basis for hierarchical histone de-β-hydroxybutyrylation by SIRT3
a Schematic diagram of histone Kbhb peptides used in ITC and deacylation assays. b ITC fitting curves of SIRT3 titrated with all histone S-form Kbhb peptides listed in a. c Schematic diagram of enzymatic kinetics parameters of histone Kbhb-S peptides catalyzed by SIRT3. N.D. not detected. d Summary of de-β-hydroxybutyrylation activities over all histone S-form Kbhb peptides listed in a catalyzed by HDAC3 (measured by MALDI-TOF MS). e Coomassie blue staining results of extracted nucleosomes from HEK293T cells. NC, nucleosome. f Immunoblotting results of deacylation assays on Kbhb-modified nucleosome by both wild type and catalytic mutant SIRT3 (SIRT3H248Y). Histone H3 and H4 were used as loading controls
