Fig. 3: [U-13C] glucose tracing shows differences in metabolite contribution to the TCA cycle and decreased de novo nucleoside phosphate synthesis upon PD-L1 inhibition. | Cell Discovery

Fig. 3: [U-13C] glucose tracing shows differences in metabolite contribution to the TCA cycle and decreased de novo nucleoside phosphate synthesis upon PD-L1 inhibition.

From: Global alteration of T-lymphocyte metabolism by PD-L1 checkpoint involves a block of de novo nucleoside phosphate synthesis

Fig. 3

a Selected isotopomers of uridine monophosphate and ribose-5-phosphate that represent the contribution of fully labeled ribose-5-phosphate (UMP M5) plus partially labeled aspartic acid (UMP M6–7). b Selected isotopomers of TCA metabolites used to infer metabolite contributions to the TCA cycle, expressed as the percentage of all isotopomers detected for the respective compounds. M0: unlabeled. M2: Two heavy carbons from glucose. M3: Three heavy carbons from glucose. c Schematic of inferred relative contributions to the TCA cycle in PD-L1-treated cells. Thick arrows represent more relative contribution of metabolites in the PD-L1-treated cells compared to the Fc control.

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