Fig. 4: PD-L1 causes a block in nucleoside phosphate synthesis at 24 h. | Cell Discovery

Fig. 4: PD-L1 causes a block in nucleoside phosphate synthesis at 24 h.

From: Global alteration of T-lymphocyte metabolism by PD-L1 checkpoint involves a block of de novo nucleoside phosphate synthesis

Fig. 4

a Relative levels of nucleoside phosphates and aspartic acid at 24 h of treatment. b Statistically significantly changing 13C incorporation into nucleoside phosphates from [U-13C] glucose via ribose-5-P, as reflected in the their M5 isotopomers. All measurements have a FDR < 0.05 but the fold-reduction is larger for pyrimidines than for purines. c Western blot of phosphorylation status of mTORC1 targets. T-cells were treated for 48 h as indicated. Jurkat cells were treated for 2 h. PHA: phytohemagglutinin 5 µg/mL. Rapa: rapamycin 20 nM. d Metabolic activity (alamar blue resazurin reduction) assay of Fc and PD-L1-treated T-cells with or without nucleoside cocktail supplementation for 48 h. Summary of three experiments performed on separated days in triplicates (n = 9 per condition).

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