Fig. 2: Expression of AMPARs and NMDARs in spine and shaft synapse at different culture stages. | Cell Discovery

Fig. 2: Expression of AMPARs and NMDARs in spine and shaft synapse at different culture stages.

From: Structure and plasticity of silent synapses in developing hippocampal neurons visualized by super-resolution imaging

Fig. 2: Expression of AMPARs and NMDARs in spine and shaft synapse at different culture stages.

a–c Example dual color STORM images of GluN2B (green) and GluA1 (red) expression in shaft (a1, b1, c1) and spine (a2, b2, c2) synapses at DIV 11, 16-17 and 21-23. Scale bar, 500 nm. d–f Scatter plots of GluN2B and GluA1 localizations in shaft (black) and spine (red) synapses in culture stages corresponding to the examples in (a–c), respectively. g–i Histogram of NGluA1/(NGluA1 + NGluN2B) in shaft (black) and spine (red) synapses in culture stages corresponding to the examples in (a–c), respectively. j–k Summary of GluN2B (green) and GluA1 (red) expression in spine synapse (j) and shaft synapses (k) at different culture stages, with Error bars are standard error of the mean (SEM), with *** denoting P < 0.001, ** denoting P < 0.01, * denoting P < 0.05, n.s. denoting no significance, t-test, in this and subsequent Figs unless otherwise noted. n = 35 (shaft), 8 (spine) in DIV11; n = 51(shaft), 151(spine) in DIV16 -17; n = 30 (shaft), 171(spine) in DIV21-23.

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