Fig. 3: Differential distribution of shaft and spine synapses along neuronal dendrites.

a Stitched conventional fluorescence images showing distal (green box) and proximal (red box) dendritic segments of a hippocampal neuron. b STORM images of the distal segments in green box of (a). A magnified view of a shaft synapse, arrow head in (b) is shown in (b1). c STORM images of the proximal segments in red box of (b). A magnified view of a spine synapse, arrow in (c) is shown in (c1). Scale bars in (a): 10 µm; (b, c): 2 µm, (b1, c1): 500 nm. d Summary of synapse density in distal (n = 21) and proximal (n = 12) segments for shaft and spine synapses.