Fig. 1: BmSXL is enriched in nuclei and distributed in a mesh-like pattern in the cytoplasm of the somatic cyst cells enveloping the spermatids during spermiogenesis.
From: Dysfunction of dimorphic sperm impairs male fertility in the silkworm
a, b Representative immunofluorescence images of spermatocysts at day four of the fifth larval instar (L5D4) stage and pupal stage day 1 (P1). c–f Representative images of elongating apyrene sperm bundles at stage P1 and P7. g, h Representative images of apyrene sperm bundles undergoing peristaltic squeezing process at stage P7. i–m Representative images of elongating eupyrene sperm bundles at stage L5D4. n Representative images of a eupyrene sperm bundle with elongating nuclei at stage P1. o Representative images of a eupyrene sperm bundle undergoing peristaltic squeezing at stage P7. p Representative images of a eupyrene sperm bundle after peristaltic squeezing at stage P7. Blue, Hoechst; red, filamentous actin (F-actin); green, BmSXL. Scale bars, 20 µm (a–p). q Average intensity distribution for BmSXL in the cytoplasm and nucleus of somatic cyst cells in the spermatocysts and sperm bundles. Between 30 and 50 cells (n = 30~50) from 10 spermatocysts or sperm bundles were analyzed for each stage. Data are mean ± SEM (****p < 0.0001, paired t-test).
