Fig. 1: The heterotrimer SMARCB1–SMARCC2 formation is required for the tumor suppression function of SMARCB1.

a Schematic representations of full-length SMARCB1 and SMARCC2. The protein fragments of the SMARCB1^(169–385)/SMARCC2^(325–518) complex used for structural determination are indicated by a two-way arrow. b Ribbon diagram representation of the SMARCB1^(169–385)/SMARCC2^(325–518) complex. c–d The interface between Rpt1 or Rpt2 of SMARCB1 and SWIRM of SMARCC2. All interaction details between SMARCB1 and SMARCC2 are shown in Supplementary Fig. S6. e Co-IP experiments. The bottom panel shows 3% of the Myc fusion proteins for each IP. f Model of SMARCB1–SMARCC1/2–SMARCA4 complex assembly. The filled black hexagon and the filled gray ellipse represent the BAF core and ATPase modules, respectively. For clarify, only SMARCB1, SMARCC1/2, or SMARCA4 are shown in the cartoon diagram. g–h Statistical graph of the percentage of BrdU positive nuclei (g) or the colony number (h) in different cell lines. Bars represent average and standard deviation of the percentage of BrdU positive nuclei or the colony number of different cell lines. The experiments were performed in triplicates in e–h. Error bars represent SEM. i Tumor growth curve of nude mice bearing BT-12 cells with inducible expression of SMARCB1 and different mutants in xenograft assay. Data represent mean ± SEM (n = 5). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.