Fig. 3: SPR characterization of the binding between ACE2s and SARS-CoV-2 RBD or SARS-CoV RBD, and ACE2s mediated pseudoviruses transduction.
From: Broad host range of SARS-CoV-2 and the molecular basis for SARS-CoV-2 binding to cat ACE2
a The mFc-tagged ACE2s in supernatants were captured by anti-mIgG Fc antibodies immobilized on the CM5 chip, and sequentially tested the binding with serially diluted SARS-CoV-2 RBD or SARS-CoV RBD. The SARS-CoV-2 NTD was used as the negative control. The raw and fitted curves were displayed in dotted and solid lines, respectively. b The binding affinities between ACE2s and SARS-CoV-2 RBD or SARS-CoV RBD are shown with the means ± SD of three independent experiments. c BHK21 cells expressing the indicated ACE2 orthologs were infected with SARS-CoV-2 or SARS-CoV pseudovirus containing luciferase-reporter. Luciferase activity was determined at 24 h post infection. Relative transduction values (%) for each ACE2 ortholog mediated pseudovirus transduction were normalized to hACE2 and presented as a heatmap according to the indicated color code. Pseudovirus transduction were performed at least twice for each ACE2 with three replicates. Data shown are representative data with the mean of three replicates.
