Table 1 Cryo-EM data collection, refinement and validation statistics of cACE2 in complex with SARS-CoV-2 RBD.
From: Broad host range of SARS-CoV-2 and the molecular basis for SARS-CoV-2 binding to cat ACE2
cACE2 in complex with SARS-CoV-2 RBD | |
|---|---|
Data collection and processing | |
Magnification | 130k |
Voltage (kV) | 300 |
Electron exposure (e-/Å2) | 50 |
Defocus range (μm) | −1.8 to −2.2 |
Pixel size (Å) | 0.99375 |
Symmetry imposed | C1 |
Final particle images (no.) | 195,370 |
Map resolution (Å) | 3.0 |
FSC threshold | 0.143 |
Refinement | |
Initial model used (PDB code) | 6LZG |
Model resolution range (Å) | up to 3 |
FSC average (model to map) | |
Whole unit cell | 0.7298 |
Around atoms | 0.74 |
Model composition | |
Non-hydrogen atoms | 6359 |
Protein residues | 792 |
Ligands | 1 |
B factors (Å2) | |
Protein | 55.1 |
Ligand | 56.7 |
R.m.s. deviations | |
Bond lengths (Å) | 0.003 |
Bond angles (°) | 0.481 |
Validation | |
MolProbity score | 2.29 |
Clashscore | 8.71 |
Poor rotamers (%) | 5.90 |
Ramachandran plot | |
Favored (%) | 96.45 |
Allowed (%) | 3.55 |
Outliers (%) | 0 |
