Fig. 1: Single-cell RNA sequencing of hPSCs-derived HPCs and adult peripheral blood HSPCs.

a Scheme of the experiment design. Fresh Lin⁻CD34⁺CD38⁻ HSPCs mobilized in human peripheral blood (PB-HSPCs) were sorted by FACS. Human ESCs were differentiated into blood lineages in a monolayer, defined condition. The floating blood cells at differentiation day 8 were sorted by CD43. The sorted cells were analyzed by 10× genomics for single-cell RNA sequencing (scRNA-seq). b t-SNE projection of PB-HSPC and hESC-HPC sample cells assigned based on samples. Each dot represents one cell and colors represent cell samples. c t-SNE projection of hESC-HPC, PB-HSPC, and PB-LPC cluster cells assigned based on clusters. Each dot represents one cell and cells are colored according to their assigned clusters. d Expressions of known hematopoietic and blood lineage marker genes at single-cell resolution. Color displays expression level (TPM, Log-scaled). e Expression profiles of top highly expressed genes in hESC-HPC, PB-HSPC and PB-LPC clusters. Color displays the scaled expression level (TPM, z-normalized) and diameter denotes fractional expression. f Violin plots show expression levels (TPM, Log-scaled) of selected hematopoietic and blood lineage marker genes with GO terms enriched in hESC-HPC, PB-HSPC and PB-LPC clusters. Colors represent clusters. g Top Gene Ontology (GO) terms enriched in hESC-HPC, PB-HSPC, and PB-LPC clusters. Colors represent clusters. The length of bar represents P value.