Fig. 6: CASK-Mint1 interaction regulates insulin secretion.
From: CASK modulates the assembly and function of the Mint1/Munc18-1 complex to regulate insulin secretion
a Co-immunoprecipitation of exogenous CASK, Mint1 and Mint1/4A muntant in 293 cells. b Co-immunoprecipitation of CASK and Mint1 in glucose-stimulated INS-1E cells that were transfected with empty vector (Con), Mint1/WT or Mint1/4A mutant. c Co-immunoprecipitation of exogenous Munc18-1 and CASK in 293 cells. Mint1/WT or Mint1/4A mutant, CASK/WT or △CaMK mutant and Munc18-1 were overexpressed in 293 cells as indicated. d Overexpression of Mint1/4A mutant inhibited insulin secretion in INS-1E cells. e Reduced insulin secretion due to RNAi targeting Mint1 (Ri-1) could be rescued by siRNA-resistant wild type Mint1 (Ri-1+WT) but not the mutant (Ri-1 + 4A).
