Fig. 4: m¹A-seq identified transcripts with altered methylation in trophoblast induced by hypoxia.

a Composite motif logo of the multiple sequences with significant m¹A peaks for normoxia (top) or hypoxia (bottom) HTR8/SVneo cells. b Number of m¹A peaks identified in m¹A-seq in normoxia and hypoxia HTR8/SVneo cells. c Number of m¹A-modified genes identified in m¹A-seq. Common m¹A genes contain at least one common m¹A peak, while unique m¹A genes contain no common m¹A peak. d Graphs of m¹A peak distribution showing the proportion of total m¹A peaks in the indicated regions in normoxia and hypoxia cells (top) and loss of existing m¹A peaks (unique peaks in normoxia) or the appearance of new m¹A peaks (unique peaks in hypoxia) after hypoxia treatment (bottom). e, f Enrichment fold of the indicated RNA transcripts in m¹A (e), YTHDF3 (f) IP vs. RNA input control. g, h Enrichment fold of the indicated RNA transcripts in m¹A (g), YTHDF3 (h) IP vs. RNA input control in HTR8/SVneo upon hypoxia for 12 h. Each transcript was quantified by qRT-PCR. NS: not significant; **p < 0.01 (Student’s t test); NS, not significant. Data are representative of three independent experiments (mean and s.d. of technical triplicates (e–h)).