Fig. 6: YTHDF3 regulated the activity of trophoblast via targeting IGF1R.
a Immunoblot analysis of the indicated proteins in HTR8/SVneo transfected with IGF1R siRNA #1 or #2 as indicated for 48 h. b, c qRT-PCR analysis of MMP9 mRNA in HTR8/SVneo transfected with the indicated siRNA (b) or plasmid (c). d, e Left: Migration (d) and invasion (e) analysis of HTR8/SVneo cells transfected with NC vs. siIGF1R by Transwell assay. Representative images are shown. Right: The number of migration and invasion cells was counted by the ImageJ software. f qRT-PCR analysis of MMP9 mRNA in stably expressing shYTHDF3 HTR8/SVneo transfected with siIGF1R. g qRT-PCR analysis of MMP9 mRNA in stably expressing YTHDF3 HTR8/SVneo transfected with IGF1R overexpression plasmid. h Immunoblot analysis of the indicated proteins in HTR8/SVneo transfected with shYTHDF3, and stimulated with IGF cytokine for the indicated time. i Working model for the mechanism of YTHDF3 inhibiting trophoblast invasion by decreasing IGF1R expression via promoting m1A-carrying IGF1R decay. P: Phosphorylation. Scale bars, 100 μm; **p < 0.01 (Student’s t test); NS, not significant. Data are representative of three independent experiments (mean and s.d. of technical triplicates (b, c, f, g)).
