Fig. 2: PGC-like tumor cells serve as a seed for liver metastasis. | Cell Discovery

Fig. 2: PGC-like tumor cells serve as a seed for liver metastasis.

From: Identification of primordial germ cell-like cells as liver metastasis initiating cells in mouse tumour models

Fig. 2: PGC-like tumor cells serve as a seed for liver metastasis.

a The percentage of PGC-like cells in paired primary tumors and cancer liver metastasis from spontaneous lymphomas in p53−/− mice, 4T1 tumors and B16-BL6 tumors. b The bigger cluster of tumor cells in liver tissues of the mice subcutaneously inoculated with lymphoma tumor cells in p53−/− mice or 4T1 cells mainly shows the differentiated tumor cells. c 4T1 cells were subcutaneously injected into BALB/c mice, and the mice were sacrificed to harvest for liver tissues for histological analysis at different time points. The percentage of PGC-like cells and tumor density in the paired liver metastasis at different time points. Liver section at different time points were stained with Oct4 antibody. d Histological analysis of liver metastasis in mice after subcutaneously injected with 4T1 and 168FARN cells, respectively. The plot shows the percentage of liver metastasis in mice after subcutaneously injected with 4T1 and 168FARN cells, respectively. e Bright field image of AP staining in 4T1 and 168FARN. The plot shows the percentage of AP-positive cells in 4T1 and 168FARN cultures. f The real-time PCR analysis of the expression of PGC-related genes in 4T1 and 168FARN cells. g The percentage of AP-positive cells in TBMDCs-2 (T2) and TBMDCs-7 (T7) cultures (left panel). The percentage of liver metastasis in mice after subcutaneously injected with TBMDCs-2 (T2) and TBMDCs-7 (T7) cells, respectively (left panel). h The real-time PCR analysis of the expression of PGC-related genes in TMDCs-2 (T2) and TMDCs-7 (T7). **P < 0.01. Scale bar = 50 μm. Scale bar = 20 μm in (b, c), 50 μm in (d, e, g).

Back to article page