Fig. 3: Anti-plasmid activity of the LdCsm system. | Cell Discovery

Fig. 3: Anti-plasmid activity of the LdCsm system.

From: Characterization of a novel type III CRISPR-Cas effector provides new insights into the allosteric activation and suppression of the Cas10 DNase

Fig. 3

a Schematic of the test plasmids pBad-G, pBad-CTR, and pBad-NTR. Gene expression from these plasmids is under the control of a L-arabinose-inducible Bad promoter. Therefore, mRNAs carrying CTR or NTR are expressed from the corresponding plasmids (pBad-CTR or pBad-NTR) in the presence of L-arabinose, but their expression is completely repressed in glucose media. b Schematic of the interference plasmid assay for determination of the LdCsm anti-plasmid activity. c Transformation efficiency data of the three different plasmids obtained from different growth media.

Back to article page