Fig. 1: Cell cycle-dependent localization of cGAS.
From: Phosphorylation of cGAS by CDK1 impairs self-DNA sensing in mitosis
a Cellular localization of cGAS in Raw264.7 cells. Raw264.7 cells were fixed and stained with anti-cGAS and DAPI. b cGAS is associated with chromosomes in different phases of mitosis. MLF cells were fixed and stained with anti-cGAS and DAPI before confocal microscopy. Inter, interphase; Pro, prophase; Prometa, prometaphase; Meta, metaphase; Ana, anaphase; Telo, telophase. c cGAS is associated with chromosomes upon NEBD in living cells. The GFP-cGAS plasmid was transfected into H2B-RFP-expressing HeLa cells for 20 h before live confocal microscopy. Scale bar, 10 μm. Data shown are representative of at least two biological repeats.
