Fig. 1: In vivo samples acquired reflecting the morphogen gradients.

a The Drosophila wing imaginal disc diagram. The expression patterns of Ci and Hh specify the A (red region) and P (blue region) compartment of wing imaginal disc, respectively. b The diagram of the adult wing. The whole adult wing is derived from the wing pouch region (red and blue colored region) in wing imaginal disc. There are five veins in the wing. Among them, the first three veins (L1–L3) are developed from the A compartment cells and the other two (L4 and L5) are from the P compartment cells. c Diagram of the wing imaginal disc section view in A compartment. The dot-line circled region is the area subjected to laser capture. d–d‴ Immunostaining of a ptcGal4-uas-GFP wing imaginal disc. GFP signals (d and green in d‴), Ptc signals (d′ and red in d‴) and Ci signals (d′ and blue in d‴). Scale bar, 50 μm. e–k′ The wing disc sections were cut along A–P axis (bright filed). The circled areas were obtained by laser capture. The wing disc sections before laser capture (e–k). The wing disc sections after laser capture (e′–k′). l–p Real-time qPCR results showing the expression levels of the indicated genes at different positions along A–P axis in a wing imaginal disc. q Workflow of Geo-RNA-seq to acquire PGE profiles along A–P axis and bioinformatics analysis.