Fig. 5: Attractor analysis in response to Hh production changes.
From: Analysis on gene modular network reveals morphogen-directed development robustness in Drosophila
a Hypothesis model of how Hh production changes affect the induction status of M8 along A–P axis. b In response to Hh production changes, the ON or OFF status of M8 at different positions is changed accordingly. Percentage of the normal attractor (attractor SN) was calculated based on the corresponding M8 changes. c The histogram of the percentage of normal attractor SN in response to Hh production changes. The vertical coordinate indicates the variations of M8 in response to different Hh levels. The asterisk indicates the physiological state of M8. The upper states of M8 representing the Hh level decrease. The lower states of M8 representing the Hh level increase. The X axis showed the percentage of SN. d–f″ Wing discs with the indicated genotypes were immunostained with anti-Ptc (blue) antibody or anti-Ci (red) antibody. Ptc and Ci signals were presented in Drosophila wing discs with WT (d–d″), Hh overexpression (hhGal4-uas-Hh) (e–e″), or Hh knockdown (hhGal4-Hh RNAiV1402) (f–f″) genotypes. The width changes of Ptc-positive regions (between two white arrows) indicate the corresponding Hh gradient alterations (d–f), which is also in accordance with the Ci change (d′, e′ and f′). g–i Morphology of Drosophila adult wings with the indicated genotypes. An adult wing of WT control (g), an adult wing of Drosophila with Hh overexpression that is driven by hhGal4 in third instar larvae stage (h), an adult wing of Drosophila with reduced Hh production that is achieved by hhGal4-Hh RNAiV1402 in third instar larvae stage (i).
